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KMID : 0378019600030110095
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New Medical Journal
1960 Volume.3 No. 11 p.95 ~ p.97
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Experimental Studies on the Influences of the Influences of a Porionged Administration of Steroid Hormones to the Blood Vessels, Blood and Parenchymatous Organs of Rabbits
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Abstract
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The endocrinologic studies of the steroid hormones are innumerable and the great advance has been made. The application of steroid hormones is an essential and common tool ill the practice of the medicine today. In view of wide application of these hormones and often the necessity of a large dose and prolonged administration of hormones to achieve and maintain their effect, the. studies of the effect of these hormones other than beneficial to the host as well as the mechanism of beneficial effect are desired.
Present studies are consisted of two parts. The first part is to investigate the effect of a large dose of seroid hormones on blood, particularly serum total cholesterol, electrolytes and formed elements, and the effect on the major blood vessels. The se. cond part is to investigate the effect on the paren. chymatous organs, namely, liver, heart, kidneys and adrenal, with special respect to the carbohydrate and lipid metabolism.
For the experiment, sixty domestic rabbits, thirty females" and 30 males, of around 1, 000 gms. were divided into four groups. Group I is consisted of twelve rabbits, 6 females and 6 males, and served as normal control; group lE consisting of 16 rabbits, 8 females and 8 males, was given 10 mg/kg. of cortisone acetate once every day.
Group III consisting of 16 rabbits, - 8 females amd 8 males, was given 4, 000 I.U./kg of estradiol benzoate once every third day; and Group IV, consisting of 16 rabbits, 8 females and 8 males, was given 20 mg./kg of testosterone propionate once every third day. All hormones were given by intramuscular injection at gluteal region. One-half of the animals from each group were killed after the administration of hormones for 45 days and the remaining one-half were killed after the adminidtration of hormones for 90 days. All animals killed were necropsied, and the tissues were fixed in 10% neutral formalin and 10% neutral formalin added with 1% calcium bichloride. All sections were routinely stained with H & E, and PAS, Sudan III, Baker´s and Schultz tests for parenchymatous organs; PAS, Sudan III, van Gieson, and Verhoeff van Gieson stain for blood vessels, were done additionally. Laboratory examinations included, urinary 17-ketosteroids, serum total cholesterol, serum electrolytes, and routine hematologic study.
The result of study showed marked elevation of serum total cholesterol in cortisone treated group and moderate depression of serum total chelesterol in testosterone treated group; marked and absolute neutrophilia, lymphopenia and eosinopenia in cor-
tisone treated group, and mild but similar trends in estrogen and testosterone treated groups. No notable alterations of serum electrolytes or blood vessels, aorta, coronaty artery, and femoral artery were observed.
The histopathologid changes of heart were consisted of accumulation of glycogen and fat, focal nee-rosis of myocardium particularly at subendocardial region, and intimal thickening of small artery in cortisone treated group, while no such changes were observed in estrogens and testosterone treated groups.
The liver showed swelling of hepatic cells, and marked accumulation of glycogen in cortisone treated group; increase of binuclear calls, glycogen infiltration, and hyperplasia of bile duct epithelial cells in estrogen treated group; and the testosterone treated group showed milder but similar changes seen in estrogen group.
Kidneys showed no notable alteration in cortisone treated group, but marked deposition of glycogen and fat in proximal convoluted tubules and glomeruli in both estrogen and testosterone treated groups.
Adrenals showed marked atrophy of the zona fasciculata of both sex in cortisone treated group, no remarkable changes in male but marked atrophy of zona reticularis and slight hypertrophy and the depletion of stainable lipids in female rabbits in estrogen treated group, and marked atrophy and de-posit of lipids in zona fascicularis of both sex in testosterone treated group.
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KEYWORD
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